Main focus of GENE QUANTIFICATION web page is to. ->optimal for Rotor-Gene 3000 or Rotor-Gene 6000 applications. The Q-Gene software application is.
Operator Manual 1.7.87. Welcome to the Rotor-Gene 6000 Series Software version 1.7. The Rotor-Gene 6000 is used in research applications that range from. The Rotor-Gene Q enables streamlined analysis for a wide range of applications. Rotor-Gene ScreenClust HRM Software f. With the Rotor-Gene 6000 you can.
Sinumerik 840d Simulator. Unique rotary design for outstanding performance The unique centrifugal rotary design of the Rotor-Gene Q makes it the most precise and versatile real-time PCR cycler currently available (see figure 'Cross-section of the Rotor-Gene Q'). Each tube spins in a chamber of moving air, keeping all samples at precisely the same temperature during rapid thermal cycling. Detection is similarly uniform. When each tube aligns with the detection optics, the sample is illuminated and the fluorescent signal is rapidly collected from a single, short optical pathway. This thermal and optical uniformity results in sensitive, precise, and fast real-time PCR analysis (see figure 'Precise real-time PCR analysis'). It also eliminates sample-to-sample variations and edge effects.
These are unavoidable in traditional block-based instruments due to temperature gradients across the block and multiple, complex optical pathways. The rotary design delivers: • Well-to-well variation ±0.02°C • Uniform detection eliminating the need for ROX reference dye • Fast ramping and negligible equilibration times for short run times • Complete confidence in your results! Unrivaled optical range enables multiple applications Whether your assay is based on intercalating dyes such as SYBR Green, probes such as hydrolysis (TaqMan), hybridization (FRET), Scorpion probes, or other multiplex chemistries, the Rotor-Gene Q meets your requirements. With up to 6 channels spanning UV to infrared wavelengths, the cycler delivers the widest optical range currently available (see Table 'Channels for optical detection').
In addition, the software allows you to create new excitation/detection wavelength combinations, which means that the Rotor-Gene Q is compatible with dyes you may use in the future. Expand your research with HRM High-resolution melting analysis (HRM) is a closed-tube, post-PCR analysis that has raised enormous scientific interest. HRM characterizes double-stranded PCR products based on their dissociation (melting) behavior. It is similar to classical melting curve analysis, but provides far more information for a wider range of applications. PCR products can be discriminated according to sequence, length, GC content, or strand complementarity, down to single base-pair changes. Previously unknown and even complex sequence variations can be readily detected and characterized in a robust and straightforward way. The rotary design of the Rotor-Gene Q and its outstanding thermal and optical performance are highly suited to HRM.